- Capping Reagent A (1-Methylimidazole in Acetonitrile, 2:8) is the base component of a two-part capping system used in automated solid-phase oligonucleotide synthesis to cap unreacted hydroxyl groups and prevent deletion sequence formation.
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Part A of two-component capping system in automated DNA/RNA oligonucleotide synthesis
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Provides the base (NMI) component that activates acetic anhydride for unreacted amine capping
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Used in solid-phase synthesis to prevent deletion sequence accumulation
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Compatible with standard phosphoramidite synthesis cycles on automated synthesizers
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Chemical Manufacturing
Product Information
| CAS | 616-47-7 (NMI) / 75-05-8 (MeCN) |
|---|---|
| Assay | 20% v/v 1-Methylimidazole in 80% v/v acetonitrile |
| Grade | Bioreagent Grade |
| Appearance | Clear, colorless solution |
| Solubility | Fully miscible with acetonitrile and DMF; avoid water |
| Autoship Available | Yes |
| Pillar | High Purity |
| Water | NMT 50 ppm H₂O |












