High Performance Liquid Chromatography (HPLC) is a well-established technology for separating, identifying, and quantifying components of a mixture in analytical practices. For the production of reagents, buffers, mobile phases, and any sample preparation, HPLC analysis needs Type I ultrapure water. The American Society for Testing and Materials (ASTM) has designated ultra-pure water as Type I on the basis of purity (<50 ppb of Total Organic Carbons), low conductivity (<0.056 μS/cm), and UV-absorbance. Type I water is required when HPLC is used in combination with a mass spectrometer (MS) for the ultra-sensitive applications. Lab Alley is selling its ultra-pure HPLC Grade water in the United States of America. Lab Alley's HPLC grade water is widely used in regular HPLC operations and Ultra High- Performance Liquid Chromatography (UHPLC) applications.
HPLC grade is highest grade of chemicals used for HPLC analysis. Pure HPLC Grade Water is a better quality than Lab Grade Water, USP Grade Water And Reagent Grade Water.
HPLC Grade Water is used in most HPLC mobile phases and in sample preparation. Because it dissolves most substances and is nontoxic, it is known as a Universal Solvent. HPLC grade water is water wherein a specific conductivity (16-18) is maintained by ELGA water machine and filtered through 0.22 micron filter to avoid the particulate matter.
Water, HPLC Grade (CAS 7732-18-5) is liquid ultrapure water prepared for high performance liquid chromatography. HPLC work needs ultrapure water for reagents, buffers, mobile phases and sample preparation, and this grade is made for that job. It’s filtered through a 0.22 micron filter to keep particulate matter out of your system, so it suits regular HPLC runs and UHPLC work alike. Water also dissolves most substances, which is why chemists call it the universal solvent. You’ll find a Safety Data Sheet, a Technical Data Sheet, a PFAS/PFOS Free Statement and Certificates of Analysis in the Product Manuals section, so you can check the paperwork before it reaches your bench.
Most people reach for this water in HPLC mobile phases, sample preparation, spectrometry, biochemistry labs and hospitals. The trouble starts when your water isn’t up to the job. Impurities can muddy your results, and chasing missing paperwork only slows you down. Lab Alley is here to take that off your plate. The SDS, TDS and Certificates of Analysis are available online, so you don’t have to wait on anyone. Here’s the plan. Pick the 4L size for everyday runs or the 4 x 4L case if you go through water quickly. Set up Autoship so it arrives before you run low, or request a quote if you need something else. We’ll keep it on budget, on time, every time. Request your quote here.
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HPLC mobile phase preparation
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Sample preparation for HPLC analysis
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UHPLC applications
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Spectrometry
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Making reagents and buffers
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Biochemistry lab work
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Hospital use
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Q: What is Water, HPLC Grade used for?
A: It’s used in most HPLC mobile phases and in sample preparation. You can also use it to make reagents and buffers for HPLC analysis.Q: What does HPLC grade mean, and is it suitable for mobile phases?
A: HPLC grade is the water grade made for HPLC analysis. Yes, it’s intended for use in most HPLC mobile phases.Q: What sizes are available, and does it ship as hazmat?
A: You can order a 4L size or a 4 x 4L case. For transport details, check the Safety Data Sheet in the Product Manuals section or ask our team before you order.Q: How do I get the SDS and Certificate of Analysis?
A: Both are listed on this page under Product Manuals, and you can open them online. The Technical Data Sheet and PFAS/PFOS Free Statement are in the same place.Q: How should it be stored?
A: Our guide on how to store water walks through it. Also follow the storage section of the Safety Data Sheet.Q: Is the water filtered?
A: Yes. It’s filtered through a 0.22 micron filter to keep particulate matter out.Q: How is it different from other water grades on the site?
A: Water grades are made for different jobs, and this one is made for HPLC work. Our Types of Water guide explains how the grades compare.
Product Information
| CAS | 7732-18-5 |
|---|---|
| Synonyms | Aqua, Deionized Water, Dihydrogen Oxide, Dihydrogen Monoxide, Purified water |
| Assay | Min 99.5% |
| PubChem ID | 962 |
| Grade | HPLC Grade |
| Color | Clear |
| Physical form | Liquid |
| Appearance | Clear |
| Odor | Odorless |
| Melting point | 0 °C (32 °F) |
| Boiling Point | 100 °C (212 °F) |
| Vapor pressure | 16 mm Hg at 20°C (68.0°F) |
| Specific gravity | 1 |
| Solubility | Fully Miscible in Water |
| Molecular formula | H2O |
| Molecular weight | 18.02 |
| Autoship Available | Yes |
| Pillar | High Purity |
| Residue after evaporation | Max 1 ppm |
| Substances Reducing Permanganate | To pass Test |
| UV Absorption @ 190nm | 0.01 max. |
| UV Absorption @ 200nm | 0.01 max. |
| UV Absorption @ 210nm | 0.01 max. |
| UV Absorption @ 250nm | 0.005 max |
| UV Absorption @ 300-400nm | 0.005 max |
| Viscosity | 0.7 cSt (40°C) |
| Water | Min 99.5% |
