Crystal Violet Dry Stain Powder ACS Grade *

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Crystal Violet Stain Powder  | ACS Grade | Gentian Violet | Blue Dry Histological & Biological Stain | Formula C25N3H30Cl | Stain For Amyloid, Colony Formation Assay, Biofilm, Cell Viability, Cell Counting, Bacteria, Mammalian Cells

Crystal Violet Dry Stain ACS (Gentian Violet) Features:

CAS Number 548-62-9
Molecular Formula C25H30ClN3
Formula Weight 407.99
Assay Min 90.0%
Loss on Drying 2.5%
Sensitivity as Indicator To Pass Test
Absorbency Characteristics To Pass Test

Crystal Violet Stain Powder (Gentian Violet Stain) Product Summary

  • Other Names: GV | Hexamethyl Pararosaniline Chloride | Crystal Violet | Methyl Violet 10B, Aniline violet, Basic violet 3, Baszol Violet 57L, Brilliant Violet 58, Hexamethyl-p-rosaniline chloride, Methylrosanilide chloride, Methyl Violet 10BNS, Pyoktanin
  • Crystal Violet Solubility: Soluble in water (50 mg/ml at 27° C), chloroform, alcohol (partially soluble), and glycerol (partially soluble). Insoluble in ether.
  • Crystal Violet Preparation For Cell Staining: For crystal violet solution you dilute 0.125g of crystal violet in 50 ml of 20% methanol. For lysing solution, dilute 5.88 g of sodium citrate in 100ml of D2O, use HCl to adjust pH to 4.2, add 100 ml of 50% ethanol. Concerning plates, the protocol is for 12-well plates, which is indicated in the first step.
  • Crystal Violet Stain Acidic Or Basic: If the color portion of the dye resides in the positive ion, as in the above case, it is called a basic dye (examples: methylene blue, crystal violet, safranin). If the color portion is in the negatively charged ion, it is called an acidic dye (examples: nigrosin, congo red).
  • Crystal Violet Staining Colony Formation Assay
  • Principles And Purpose: Crystal Violet Assay for Determining Viability of Cultured Cells
  • Dye Color: When dissolved in water, the dye has a blue-violet color.
  • Ingredient For Equine Herbal Products To Treat Digestion, Performance, Respiratory Issues And Thrush
  • Molar Mass‎: ‎407.99 g·mol−1
  • ATC Code‎: ‎D01AE02‎ (WHO) ‎G01AX09‎ (WHO)
  • Chemical Formula‎: ‎C25H30ClN3
  • Melting Point‎: ‎205 °C (401 °F; 478 K)
  • CAS Number: 548-62-9

Information On Gentian Violet From The US National Library of Medicine National Institutes of Health (NCBI)

Gentian Violet: A 19th Century Drug Re-Emerges in the 21st Century

Gentian violet (GV) has a long and varied history as a medicinal agent. Historically used as an anti-bacterial and anti-fungal, recent reports have shown its utility as an anti-typranosomal, anti-viral and anti-angiogenic agent.

Information About Crystal Violet From Wikipedia

Crystal violet or gentian violet (also known as methyl violet 10B or hexamethyl pararosaniline chloride) is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Crystal violet has antibacterial, antifungal, and anthelmintic properties and was formerly important as a topical antiseptic. The medical use of the dye has been largely superseded by more modern drugs, although it is still listed by the World Health Organization.

The name gentian violet was originally used for a mixture of methyl pararosaniline dyes (methyl violet), but is now often considered a synonym for crystal violet. The name refers to its colour, being like that of the petals of certain gentian flowers; it is not made from gentians or violets.

Information On Gentian Violet From PubChem

Crystal violet is an organic chloride salt that is the monochloride salt of crystal violet cation. It has been used in creams for the topical treatment of bacterial and fungal infections, being effective against some Gram-positive bacteria (notably Staphylococcus species) and some pathogenic fungi (including Candida species) but use declined following reports of animal carcinogenicity. It has also been used for dying wood, silk, and paper, as well as a histological stain. It has a role as a histological dye, an antiseptic drug, an antibacterial agent, an antifungal agent and an anthelminthic drug. It contains a crystal violet cation. Hexamethyl-p-rosaniline chloride is a green to dark green powder. 

Gentian Violet is a blue, aniline-derived dye with antifungal and antimitotic properties. Gentian violet (GV) dissociates into positive (GV+) and negative ions (Cl-) that penetrate both gram-positive and gram-negative bacterial cells. The GV+ ions interact with negatively charged components of the bacterial cell wall including lipopolysaccharide, peptidoglycan and DNA. This agent is also a mutagen and mitotic poison. GV elicits a photodynamic action mediated by a free-radical mechanism. Furthermore, this agent dissipates the action potential on prokaryotic or eukaryotic membranes by inducing permeability, thereby leading to respiratory inhibition and subsequent cell death.

Crystal Violet/ Gentian Violet Uses

Basic violet 3 is a color additive that functions as nonoxidative hair colorants. Under the US Food and Drug Administration (FDA) voluntary cosmetic registration program (VCRP), manufacturers provide information on their use of individual cosmetic ingredients as a function of product type. No current uses of any of these ingredients are reported in the VCRP. Basic Violet 3 also is used as an acid-base indicator (pH 0, green; to pH 2.0, blue), a bacteria stain, and an antiseptic. It is classified as a class I medical device for its use as a dye and stain.

Crystal violet is used in the office equipment industry for the production of indelible pencils /SRP: former/, for making stamping and hectographic inks, and for dyeing paper. The color strength in hectographic coloring matter is particularly high if crystal violet is present as the nitrate. The grain hardness of crystal violet or methyl violet is lowered by crystallizing the dye in the presence of poly(ethylene glycol). Crystal violet can also be used as a component of navy blue and black dyes for polyacrylonitrile fibers. Ink jet inks containing crystal violet and di(ethylene glycol) butyl ether have high antibacterial activity.

Acid-base indicator, alcohol denaturant, biological stain, textile dye. Used industrially to prepare inks, and to both dye and surface-coat paper. Principal use as a stain for bacteria (Gram's stain), chromatin, Holzer's stain for neuroglia. Veterinary drug for the treatment of ornamental fish infected with the parasitic protozoa Ichthyophthirius multifiliis.

Crystal Violet First Aid Information From PubChem

EYES: First check the victim for contact lenses and remove if present. Flush victim's eyes with water or normal saline solution for 20 to 30 minutes while simultaneously calling a hospital or poison control center. Do not put any ointments, oils, or medication in the victim's eyes without specific instructions from a physician. IMMEDIATELY transport the victim after flushing eyes to a hospital even if no symptoms (such as redness or irritation) develop. SKIN: IMMEDIATELY flood affected skin with water while removing and isolating all contaminated clothing. Gently wash all affected skin areas thoroughly with soap and water. If symptoms such as redness or irritation develop, IMMEDIATELY call a physician and be prepared to transport the victim to a hospital for treatment. INHALATION: IMMEDIATELY leave the contaminated area; take deep breaths of fresh air. If symptoms (such as wheezing, coughing, shortness of breath, or burning in the mouth, throat, or chest) develop, call a physician and be prepared to transport the victim to a hospital. Provide proper respiratory protection to rescuers entering an unknown atmosphere. Whenever possible, Self-Contained Breathing Apparatus (SCBA) should be used; if not available, use a level of protection greater than or equal to that advised under Protective Clothing. INGESTION: DO NOT INDUCE VOMITING. If the victim is conscious and not convulsing, give 1 or 2 glasses of water to dilute the chemical and IMMEDIATELY call a hospital or poison control center. Be prepared to transport the victim to a hospital if advised by a physician. If the victim is convulsing or unconscious, do not give anything by mouth, ensure that the victim's airway is open and lay the victim on his/her side with the head lower than the body. DO NOT INDUCE VOMITING. IMMEDIATELY transport the victim to a hospital.

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  • Hello I am looking for some dyes: Crystal violet Patent Blue Erythrosine Rose bengal and wonder to confirm if you sell it for non commercial use as well and if you have global shipment options (Czech Republic) Thanks & regards Anton

    We ship only in US and Canada. 

    Thank you.