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Alongside spectroscopy, chromatography is probably one of the main instrumental analytical techniques used in modern laboratories. There are many different types of chromatography – but they all follow the same essential principles. One of the main forms of chromatography used in laboratories is HPLC, and HPLC is commonly combined with spectroscopy in the form of a UV detector – giving rise to the powerful technique HPLC-UV.
Acetonitrile is a colorless liquid with a faint scent resembling that of a sweet fruit, with the chemical formula of CH3CN. It is the simplest organic nitrile. Acetonitrile has a boiling point between 81.3 to 82.1 °C, a molecular weight of 41.053 g·mol−1, and a melting point between −46 to −44 °C. The density of acetonitrile is 0.786 g/cm³.