Toluidine Blue Dye (Stain) *

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1 Gallon
Sale price $30.00
Sale price $581.00
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Tolonium chloride, Toluidine Blue, Toluidine Blue O, Basic Blue 17, Blutene chloride
Laboratory Grade

Toluidine Blue Dye, also known as Tolonium Chloride and Toluidine Blue Penetrant Solution has the chemical formula C15H16ClN3S. It appears as an odorless, blue-purple liquid and is miscible with Water at ambient conditions. Lab Alley’s Toluidine Blue Dye, Stain contains 0.05% Toluidine Blue O, 0.5% Triton^R X-100, and 99.46% by weight of Water. Chemically pure or Laboratory reagents are two terms often used to describe Lab Grade chemicals. Lab Grade chemicals do not meet any accepted quality or purity requirements such as the ACS Grade, the USP Grade, and the FCC Grade, despite their acceptable purity. In the United States of America (USA), Lab Alley is selling its high-quality Toluidine Blue Dye, Stain, Lab Grade online at Due to its high purity Toluidine Blue Dye, Stain, Lab Grade is highly recommended for laboratory testing.

Toluidine Blue Dye (Stain) | Dye Penetrate Solution (0.05% Toluidine Blue/0.05% Triton) | ToB

Toluidine Blue Dye Product Summary

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Toluidine Blue Test

Toluidine blue stain is used as a marker to differentiate lesions at high risk of progression in order to improve early diagnosis of oropharyngeal carcinomas. Read more here.

Is Toluidine Blue A Simple Stain?

Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. It has found wide applications both as vital staining in living tissues and as a special stain owing to its metachromatic property. Read more here.

Information About Toluidine Blue Stain From Wikipedia 

Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components. It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for Aceto-orcein stain. It is used in forensic examination, renal pathology and neuropathology. Read more here.

The results depend on the studied organs: 

  • Mastocytes in purple
  • Cartilage in purple
  • Mucins in purple/red
  • Nuclei in blue

Toluidine Blue 0.05% Vital Staining for Diagnosis of Ocular Surface Squamous Neoplasia in Kenya

Several dyes are used extensively in ophthalmic surgery. Toluidine blue (ToB) is an acidophilic metachromatic dye that stains abnormal tissue dark royal blue by penetrating into the nuclei of cancerous cells where it has a selective affinity for nucleic acids and by accumulating in the intercellular spaces. Read more here.

What Is Toluidine Blue Stain Used For? 

Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. Use of toluidine blue in tissue sections is done with the aim to highlight components, such as mast cells granules, mucins, and cartilage. Read more here.

Why Is Toluidine Blue Useful For Staining Celery? 

The use of the stain toluidine blue provides a colour difference between lignified and non-lignified cell walls, clearly highlighting specialised cells and one adaptation they have. This experiment provides a quick and eye-catching way to teach about the vascular tissue in plants and the structure of plant stems. Read more here.

Is Toluidine Blue Toxic?

It is an eye irritant. Its toxicity has not been quantified but has proven to be highly toxic to laboratory animals when injected intravenously. Other dyes in this group are known to be highly toxic, e.g. methylene blue. Read more here.

Effects of toluidine blue and destaining reagents used in sexual assault examinations on the ability to obtain DNA profiles from postcoital vaginal swabs.

Toluidine blue is an important tool to detect and document genital and perianal injuries following sexual assault. Application of toluidine blue dye and its subsequent removal from unstained areas by means of a destaining reagent, such as diluted acetic acid or a lubricant has been shown to increase the detection rate of posterior fourchette lacerations from 16% to 40% in adult rape victims. Currently, limited information on toluidine blue positive findings in sexually active control groups imposes some limitation on the interpretation of these injuries. Read more here.

Toluidine Blue Test Used For Which Cells?

The strongly acidic macromolecular carbohydrates of mast cells and cartilage are coloured red by the blue dye, a phenomenon called metachromasia. Alkaline solutions of toluidine blue are commonly used for staining semi-thin (0.5 to 1 μm) sections of resin-embedded tissue. Read more here.

Use Of Toluidine Blue In Dentistry

Toluidine blue is a cationic metachromatic dye that may selectively bind to free anionic groups such as sulfate, phosphate, and carboxylate radicals of large molecules (6). It has been used for decades as an aid to the identification of mucosal abnormalities of the cervix as well as in the oral cavity. Read more here.

The toluidine blue test: An aid in the diagnosis and treatment of early squamous cell carcinomas of mucous membranes

Early diagnosis of squamous cell carcinoma on mucosal surfaces can be lifesaving, and examination of mucosal sites should be included in the general dermatologie examination. Difficulty in distinguishing the erythematous lesions appropriate for biopsy and treatment can be partially overcome with the use of a supravital stain well known to oral surgeons. This paper recommends the addition of this test to the dermatologist's armamentarium. Five patients with mucosal in situ squamous cell carcinoma were treated. Three had squamous cell carcinoma of the glans penis, one of the vulva, and one of the oral mucosa. In all patients the toluidine blue test was helpful in the diagnosis of subclinical malignant epithelium, in the delineation of the lesion borders as a guide for surgery, and, postoperatively, in the detection of early disease recurrence. The toluidine blue test, which is a simple procedure accessible to all dermatologists, can help achieve early diagnosis and effective treatment of mucosal squamous cell carcinoma. Read more here.

Toluidine Blue Staining Identifies High-Risk Primary Oral Premalignant Lesions with Poor Outcome

There is a pressing need for the development of visual aids that will facilitate the detection of oral premalignant lesions (OPLs) with a high-risk of progression. Preliminary data suggest that toluidine blue stain may be preferentially retained by OPLs with high-risk molecular clones. In this study, we monitored OPLs from 100 patients without any history of oral cancer for an average of 44 months in order to evaluate the association of toluidine blue status with clinicopathologic risk factors, molecular patterns (microsatellite analysis on seven chromosome arms: 3p, 9p, 4q, 8p, 11q, 13q, and 17p) and outcome. Toluidine blue–positive staining correlated with clinicopathologic risk factors and high-risk molecular risk patterns. Significantly, a >6-fold elevation in cancer risk was observed for toluidine blue–positive lesions, with positive retention of the dye present in 12 of the 15 lesions that later progressed to cancer (P = 0.0008). This association of toluidine blue status with risk factors and outcome was evident even when the analysis was restricted to OPLs with low-grade or no dysplasia. Our results suggest the potential use of toluidine blue in identifying high-risk OPLs. Read more here.

Common Uses
  • Reagent
  • Dye
  • Histology applications
  • Clinical applications
Safety and Handling

Please contact us to request a Safety Data Sheet (SDS) and Certificate of Analysis (COA) for Dye Penetrate Solution (0.05% Toluidine Blue/0.05% Triton).

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Questions & Answers

  • This dye may be useful for medical device packaging. Would you classify this as a dye for nonporous packaging or porous packaging?

    As a dye it might be effective to the porous  substrate than non-porous. 

    Thank you.