TAE Buffer Solution 1 Liter


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1 Liter
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TAE Buffer Solution | 1 Liter | Triethylamine Citrole Buffer For CM75 | Tris-acetate-Edta

TAE Buffer Formula, Purpose And Composition From Wikipedia

TAE buffer is a buffer solution containing a mixture of Tris base, acetic acid and EDTA (Ethylenediaminetetraacetic acid). In molecular biology it is used in agarose electrophoresis typically for the separation of nucleic acids such as DNA and RNA. It is made up of Tris-acetate buffer, usually at pH 8.3, and EDTA, which sequesters divalent cations. TAE buffer is commonly prepared as a 50X stock solution for laboratory use. A 50X stock solution can be prepared by dissolving 242 g Tris base in water, adding 57.1 ml glacial acetic acid, and 100 ml of 500 mM EDTA (pH 8.0) solution, and bringing the final volume up to 1 litre. Read more here.

Information On Tris From Wikipedia

Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2. It is extensively used in biochemistry and molecular biology as a component of buffer solutions such as in TAE and TBE buffers, especially for solutions of nucleic acids. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g. condensations with aldehydes. In medicine, tromethamine is occasionally used as a drug, given in intensive care for its properties as a buffer for the treatment of severe metabolic acidosis in specific circumstances. Some medications are formulated as the "tromethamine salt" including hemabate (carboprost as trometamol salt), and "ketorolac trometamol". Read more here.

Information On Ethylenediaminetetraacetic Acid (EDTA) From Wikipedia

Ethylenediaminetetraacetic acid (EDTA), also known by several other names, is a chemical used for both industrial and medical purposes. It was synthesized for the first time in 1935 by Ferdinand Münz. It is an aminopolycarboxylic acid and a colorless, water-soluble solid. Its conjugate base is ethylenediaminetetraacetate. It is widely used to dissolve limescale. Its usefulness arises because of its role as a hexadentate ("six-toothed") ligand and chelating agent, i.e., its ability to sequester metal ions such as Ca2+ and Fe3+. After being bound by EDTA into a metal complex, metal ions remain in solution but exhibit diminished reactivity. EDTA is produced as several salts, notably disodium EDTA, calcium disodium EDTA, and tetrasodium EDTA (typically as the hydrate). Read more here.

Please contact us to request a Safety Data Sheet (SDS/MSDS) and Certificate of Analysis (COA) for TEA (triethylamine) Citrole Buffer for CM75.

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