How To Make 70% Ethanol From 100% Ethanol (200 Proof)
70% ethanol solutions are used in homes for cleaning, for manufacturing medicinal products and in laboratories as chemical reagents. A good recipe for a 70% non-denatured ethanol solution is to blend 70 milliliters of pure ethyl alcohol and 30 milliliters of distilled or DI water (deionized water). This is 100 milliliters of solution which is about 3.4 ounces. You can buy 70% ethanol (140 proof ethyl alcohol) solutions online from companies like Lab Alley. However, it is helpful to know how to make your own by using 100% ethanol (200 proof alcohol). Housekeepers, custodians and lab technicians dilute 100% alcohol down to 70% to use for cleaning up contaminants, sanitizing bathrooms, killing microbes in schools and sterilizing laboratory cleanrooms. A 70% alcohol solution is actually better for disinfecting than higher concentrations such as 200 proof (100%) or 190 proof (95%). 70% ethanol is bactericidal. 70% ethanol solutions are used in laboratories, research settings, healthcare facilities, industrial applications and production processes as a reagent and cleaner. In microscopic anatomy settings, a 70% ethanol solution is used for histology tissue preparation. This solution is used in microbiology labs to cold wash pellets during RNA and DNA extraction, analysis and precipitation. High-quality DNA and RNA forms the basis of genomic and genetic investigations. 70% Ethanol is commonly used as an antiseptic in laboratory environments. It has the highest effective concentration at 70% compared to 95% or 100%. This solution is ideal for sterilizing laboratory workbenches and electronics lab benches. It is perfectly suited for formulating alcohol-based hand sanitizers.
Making A 70% Ethanol Solution For Cleaning And Disinfecting
70% Ethanol For Herbal Tinctures And Extracts
Use Pure Ethanol To Make 70% Ethanol Solutions
Ethanol is a primary alcohol and known as Ethyl Alcohol, Pure 200 Proof Non-Denatured Food Grade Ethanol, Pure Alcohol, Absolute Alcohol, or Pure Grain Alcohol.
200 Proof Non-Denatured Ethyl Alcohol contains ≥ 99% contents of pure ethanol. It is highly volatile and appears as a colorless liquid with a pungent taste at room temperature. Non-denatured ethanol is deemed safe for human consumption since there are no added toxic chemicals. This makes it the best choice when choosing the right ethanol for disinfectant purposes that will be in contact with food surfaces and highly trafficked areas.
Why 100% Ethanol Is Used For Dilution?
Why Is 70% The Desired Concentration?
70% ethanol kills organisms such as viruses and bacteria by denaturing their proteins and dissolving the lipids. As a disinfectant, higher concentrations of alcohol become less effective at killing pathogens. In a study conducted by the CDC, they found that the use of 99% alcohol results in “immediate coagulation of surface proteins and prevent passage of the alcohol into the cell.” This means that when the outer cell wall becomes coagulated, it protects the virus or bacteria by preventing ethanol from moving into the cell and destroying it. Additionally, 99% ethanol evaporates rapidly which does not allow time for it to penetrate the cell wall, thus rendering it ineffective against pathogens. Use of 70% alcohol as a disinfectant is advantageous since coagulation of the cell wall proteins proceed at a slower rate, allowing the alcohol to enter the cell. The dilution with DI water is essential to denaturing proteins, and due to the concentration difference between the outer and inner cell wall, 70% ethanol can enter the cell wall and destroy both enzymatic and structural proteins. Thus, this increases the potency of the antibacterial properties.